《植物生理学报》 2011, 47(1): 37-48

植物半胱氨酸合成及调控研究进展

王小芳¹, 杨玲娟¹, 董晓宁¹, 李志孝², 焦成瑾^1,*

1 天水师范学院生命科学与化学学院, 甘肃天水 741001; 2 兰州大学化学化工学院功能有机分子化学国家重点实验室, 甘肃 兰州 730000

通信作者：焦成瑾；E-mail: jiaochj2009@163.com；Tel: 0938-8367717

摘　要：

硫是植物重要的营养元素。植物将氧化态硫吸收并还原后, 首先合成半胱氨酸使其进入各种代谢途径。合成半胱氨 酸的两种酶——丝氨酸乙酰转移酶和O-乙酰丝氨酸硫醇裂合酶均由多基因家族编码, 并能可逆的结合形成二酶复合物进 行有效的合成调节。本文对近年来半胱氨酸合成相关酶表达、定位、活性调控及转基因效果研究进展作了简要介绍, 并对 将来需要重点研究的方面作了展望。

关键词：半胱氨酸合成; 丝氨酸乙酰转移酶; O-乙酰丝氨酸硫醇裂合酶; 半胱氨酸合成酶复合物

收稿：2010-10-21   修定：2010-12-01

资助：甘肃省教育厅科研项目(1008B-07) 。

Advancement in Research on Synthesis and Regulation of Cysteine in Plants

WANG Xiao-Fang¹, YANG Ling-Juan¹, DONG Xiao-Ning¹, LI Zhi-Xiao², JIAO Cheng-Jin^1,*

¹School of Life Science and Chemistry, Tianshui Normal University, Tianshui, Gansu 741001, China; ²State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China

Corresponding author: JIAO Cheng-Jin; E-mail: jiaochj2009@163.com; Tel: 0938-8367717

Abstract:

Sulfur is an essential element that is taken up by plants in the oxidation state form, reduced to H₂S, and first incorporated into cysteine before involving metabolic processes. Cysteine synthesis occurs through two sequential reactions catalyzed by serine acetyltransferase (SAT) and O-acetylserine(thiol)lyase (OAS-TL), both of which are encoded by multigene families, and reversibly form a dienzyme complex to play a regulatory role. In this review, we summarize the recent progress made in the understanding of Cys synthesis, including the isoform expression, localization, activity regulation as well as efficiency of genetic transformation. Finally, aspects of the particularly important research in the future are suggested.

Key words: Cys synthesis; SAT; OAS-TL; CSC